Effects of Lactobacillus acidophilus and Lampung Robusta Coffee Extract on Shigella flexneri Induced Balb/C Mice; A Review of Colon Histopathology and Lymphocytes

Diarrhea is a disease that still health problem worldwide; one of the causes Shigella flexneri. Non-dairy probiotic functional foods and beverages are gaining popularity, with researchers seeking to assuage consumer concerns regarding lactose intolerance veganism. Coffee has anti-inflammatory antibacterial properties. This study aims determine effect mixture Lampung Robusta coffee extract Lactobacillus acidophilus bacteria on colon BalbC mice experience diarrhea after being induced by Twenty 3- 4- month-old weighing 35–50 grams were randomly divided into three treatment groups (125mg/kg BW, 250 mg/kg 500 BW) control group. The positive 6x108 suspension using sonde technique. negative group was not treated. Mice experienced twice in 24 hours induction. Each given 0.4 ml 0.5 1.5x108 for five days. On day 8, taken processed histologically, hematoxylin-eosin staining performed. Inflammation, bleeding, congestion, necrosis observed. showed significant differences each parameter (P < 0.05). Lymphocytes only found around inflammatory cells, small amount control, 125 500mg/kg BW treatments. However, BW.&nbsp